Generator

Part:BBa_K408000:Design

Designed by: Pavan Choppakatla   Group: iGEM10_IIT_Delhi_1   (2010-11-05)


GFP with signal peptide for hemolysin system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
    Illegal SpeI site found at 953
    Illegal PstI site found at 835
    Illegal PstI site found at 868
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 953
    Illegal PstI site found at 835
    Illegal PstI site found at 868
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
    Illegal SpeI site found at 953
    Illegal PstI site found at 835
    Illegal PstI site found at 868
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
    Illegal SpeI site found at 953
    Illegal PstI site found at 835
    Illegal PstI site found at 868
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 670


Design Notes

Apart from creating the fusion so that the stop codon is avoided and the frame does not shift, one of the Biobrick restriction sites PstI is also present in the part.


Source

Created from chimeric PCR using BBa_E0840 and the hemolysin determinant from the plasmid pANN202-812 obtained from the Karolinska Instituet, Solna Sweden. The determinant is the same as present in pHly152 which was created from wild type E.coli.

References